Techniques in Immunocytochemistry Essay Example | Topics and Well Written Essays - 250 words

Techniques in Immunocytochemistry - Essay Example After that, both slides were incubated in a medium containing diaminobenzidine (DAB). The sections were mounted with a minimal volume of the aqua mount and were then visualized under a light microscope. The tissue that was incubated with primary antibody was given a blue nucleus with a brown colored background; this indicates the presence of the target antigen (Griffins, 2011). Immunocytochemistry is a common laboratory technique that makes use of antibodies to target specific antigens in a cell via specific epitopes (an epitope is the part of an antigen that is recognized by antibodies). These bound antibodies can then be detected by using many different methods. The antigen is bound by a primary antibody, which is then amplified by use of a secondary antibody. The secondary antibody can be an enzyme that is conjugated. Immunocytochemistry binds antibodies that are specific reagents and allow unique detection of proteins and molecules. It is a valuable tool for the determination of cellular content from individual cells (Gillian et al., 2011). The tissue sections of the cardiac myosin were cut and fixed in acetone. After this, the slides were unwrapped and each slide was placed in a plastic petri-dish. The two plastic Petri-dishes were labeled; one was the control and the other the antibody. A piece of paper was put underneath each slide to form a humidity chamber. The tissue sections were blocked by adding two drops. A PBS buffer containing three percent BSA was introduced and then the tissue incubated at room temperature for five minutes. After that, the excess medium was tipped and the zone around the tissue section was wiped dry with paper toweling.  

Software Piracy Essay -- History, Dan Sokol

Many years have passed since the first pirates emerged on the sea, but they still exist and have extended their scope of action. Meaning of the word ‘’pirate’’ has changed and now can be defined as the person who illegally makes a copy of someone’s property. Music, films, books and software on hard media can be pirated by consumers. According to Gopal and Sanders (1998 381) software piracy is the operation of simulating original software which is against the law for any purpose. Such illegal acts could seriously affect industry and society. For instance, losses of software industry profits because of piracy were $12.8 billion in 1993 (Austin American-Statesman 1994), it shows that a large number of software products were pirated. Therefore, piracy is a major problem for the advance of the software industry and consumers. This essay will cover causes and effects of software piracy. There are four main reasons why the piracy of software is easy and wid espread, economic development, legislation, reliability of pirated copies and consumer’s attitude. Software piracy has the intellectual impact on society and the financial effect on the industry. This essay also will provide the combat process and analyze some proposed solutions in order to determine the effective solution. The history of software piracy starts from 1975, when Dan Sokol, who was a member of Homebrew Computer Club, copied the version of the programming language BASIC (Leinss n.d.). Since then, piracy seems to have increased by years. For instance, Givon et al. (1995 29) state that 40% of software was pirated in the United States in 1995. However, as Gopal and Sanders (1998 381) inform the percent of illegal copies there, had risen to 70% in 1997. Italy and Spain had ... ... pirated copies and consumer’s attitude were chosen as the root causes. Software piracy problem caused by integration of these factors, for dealing with this problem all causes should be eliminated. Software piracy considered as a problem, because it has a harmful effect for industry and users. It was found that there were some attempts to avoid this issue, but none of them was totally effective. Software piracy is the long-term problem of software industry and solutions of it are very controversial. To reduce the rate of piracy government should think about the level of economy and new legislations, monopolies should experience other protection devices, and consumers should think more about consequences of their choice. However, market and society adapted to existing of software piracy, and piracy seems to be not always bad, because of some benefits for industry.

The Catcher in The Rye: Depression Catcher

American Lit 12, March 2012 Depression Catcher Do you have helpless outlook on your life? One minute it's an outburst of anger. The next you're crying uncontrollably. Do you need help? The Catcher In The Rye is a novel based of the main character’s point of view, his name is Holden Caulfield. Set in 1950s New York and California, where Holden is a mental hospital telling us, the readers, about his few days after leaving Pency. The movie Ferris’ Bullers Day Off , also set in Chicago, is a movie based in the 1980s. Ferris makes his friends skip school and run all around town trying to make Cameron have fun.In both the film and novel, you see many examples of depression and suicidal thoughts from both Holden and Cameron. Teenagers face a lot of pressures, from puberty to questions about who they are and where they fit in. In The Catcher In The Rye, Holden runs away from his fancy high school, Pency, 3 days before break begins. He felt isolated with no friends. â€Å"I alm ost wished I was dead† (48). Holden had just gotten in a fight with his roommate, Stradlater. Now Ackley was trying to have a conversation about the fight with Holden but he keeps talking nonsense to Ackley.In Ferris’ Buellers Day Off, Cameron, Ferris’ best friend always seems to be sick. His family isn’t really in his life and when they are, they seems to only bring him down. In the beging scene of Cameron, he is in bed acting like he’s dying. Holden says: â€Å".. she wouldn't've been the ones that answered the phone. My parents would be the ones. So that was out† (pg. 59). He doesn’t seem to have a great relationship with his parents either. Holden wants to talk to his little sister Pheobe or anyone for that matter. He feels isolated within himself which makes him depressed.Cameron is the same in that he is very awkward and no one really seems to want to be friends with him. Towards the end of the book, Holden takes a visit to see P heope but is unable to find her. He looks in the park and museum. â€Å"The best thing, though, in that museum was that everything always stayed right where it was. Nobody'd move. You could go there a hundred thousand times, and that Eskimo would still be just finished catching those two fish, the birds would still be on their way south, the deer would still be drinking out of that water hole, with their pretty antlers and their pretty, skinny legs, and that squaw with the naked bosom ould still be weaving that same blanket† (pg. 121). Therefore, Holden would love to live in a world where everything stays frozen, where nothing changes. This way, Holden can never grow up to be an adult. Cameron is very mad after they got the car back because of all the miles that were added. He goes in to shock and is unable to move. Ferris and Sloan try their best to get him out of it but he won’t budge. While at the pool, Cameron falls in, almost committing suicide. Ferris jumps in to save.Cameron starts to laugh saying â€Å"I got you good†. Holden goes home to find Pheobe. Phoebe is the only person Holden seems to actually like and have a stable friendship throughout the novel. She is the only one who tries to push him to do better: â€Å"You don't like any schools. You don't like a million things† (pg. 169). When Holden hears this, he becomes upset and states: â€Å"`I do! That's where you're wrong-that's exactly where you're wrong! Why the hell do you have to say that†Ã¢â‚¬Ëœ I said. Boy, was she depressing me. Holden and Cameron are depressed teenagers who have suicidal thoughts because of depression. Depression: severe despondency and dejection, accompanied by feeling of hopelessness and inadequacy, a condition of mental disturbance, typicaly with lack of energy and difficulty in maintaining concentration or interest in life. People who are depressed don’t look for a way out. They have zero motivation and are always second guess ing stuff. They think the only way out is death; suicide. Just like what Holden and Cameron seem to feel.

Images of the Nude Male in European Art Essay - 706 Words

The human body, especially the nude male figure, has been a subject of art since the first scratching appeared on cave walls. Stick figures with phalluses denoted the male, procreation and dominance. Archeologists have found evidences proving that the male nude appeared in Egypt in the early Dynastic periods and in early Mesopotamia four thousand years ago. The Greek made the nude into art, and we, the Americans, made it into something commonly referred to as pornography. Until today, nude is still a major subject in art that everyone would have seen a few times, either in display cases of a museum or in greater numbers of male underwear ads. Myself being a boy of the old capital of Vietnam, I grew up with such statues in the†¦show more content†¦In Greek culture, the image of Apollo was believed an archetypal idea for male physical beauty. Unsurprisingly, his female counterpart was Venus. The statue was found in Rome during the 15th century, and you can see the pose is repeatedly used in painting and sculpture over centuries. Many fashion designers are favoring this pose, languid leaner, exists in classical art alongside more heroic models of male beauty (Bordo 406). Apollo stands there solidly, with left hand raising high; his body projects strength, solidity; hes no male waif (Bordo 392). He calmly looks straight at you as i f daring you to offend him. And of course, his penis is invisible, a symbol of malehood. Unlike the Romans, the Greek had no problem with getting naked. They did not cover their private parts with a leaf or clothes, they showed it all. One of the most famous statues of all time is the statue of David of Michelangelo. David stands 13 feet 5 inches high. The creation of the piece happened during the Renaissance, the rebirth of classical ideas. At that time, Florence needed a centerpiece to represent the city. The colossal piece of marble had been awaiting a sculptor who could do something with it for many years. Inside this piece of marble, Michelangelo saw young and powerful David. The image of David was chose to reflect theShow MoreRelatedChapter 3 Summary, Ways of Seeing Essay705 Words   |  3 Pagesof his book, â€Å"Ways of Seeing†, John Berger argues that in western nude art and present day media, that women are largely shown and treated as objects upon whom power is asserted by men either as figures in the canvas or as spectators. Berger’s purpose is to make readers aware of how the perception of women in the art so that they will recognize the evolution of western cultured art. Berger begins by claiming that in nude art the â€Å"presence† of a man is that of an actor who asserts his power overRead MoreImpact Of Pop Art1448 Words   |  6 Pagesâ€Å"THE MALE GAZE AS DEPICTED IN TOM WESSELMANN’S POP ART† Christopher Zacherl ARTH 3340: Art of the United States November 20, 2017 In the 1960s, pop art represented the attempt to return to a more objective, universally acceptable form of art following the dominance of the highly personal Abstract Expressionism in the United States and Europe. It was considered very radical compared to what the art world had seen in the 40s and 50s, rejecting the supremacy of the â€Å"high art† of theRead MoreThe Period Of Greek Art866 Words   |  4 PagesMidterm Throughout the times in Greek art, Archaic, Classical and Hellenistic periods have changed overtime from the sculptures in the form, style, and symmetry. The Archaic period lasted from 700 to 500 BCE and the sculptures haven’t yet mastered in sculpting showing realism. Their style of sculpting was similar to the Egyptians in the way that they made the bodies of the sculptures rigid with both arms on their sides and with a foot stepped forward (Greek Archaic Art). By the end of the Archaic periodRead MoreEssay on Advertisement: Greek Statue and Perfume1462 Words   |  6 Pageseyes catch an image of a nude man holding a clothed woman. The man has a muscular body and is effortlessly supporting the woman whos body is arched backwards, her arms hang in a swan-like manner. On the ground by her left foot lays a paint palette and her right hand is grasping a paint brush. The room that they are in appears to be a studio with press board floors, brick walls, and old unfinished wooden workbenches draped in cloth. The woman is painting a canvas with the image of the nude man. The foregroundRead MoreArt Critique : The Painting Of The Mi a Will Be Portrait Of Catherine Coustard 764 Words   |  4 PagesKarina Silverstein Philosophy of Art 11/18/2015 Art Critique The paintings that I will be talking about from the MIA will be Portrait of Catherine Coustard (1673-1728), by Nicolas de Largilliere, Diogenes (17th century), by Johann Carl Loth, LES FLANEUSES (2008) by Ghada Amer, and Portrait of Lucia Wijbrants (1638-1719), by Gabriel Metsu. My critique will be from the perspective of Berger. when captions are added to a painting, it impacts the viewer and changes the meaning of the painting itselfRead MoreFeminism in the Western World1342 Words   |  5 Pagescontrol of their own body image or if this idea of female empowerment is actually an illusion. BODY In order to discover the position of the current power balance regarding female body image it is necessary to review it within a historical context. Within the history of art the gender power imbalance is most startling especially in reference to the nude. Berger(1972) describes the European oil paintings of the Renaissance period as created for the pleasure of the male spectator. Paintings of thisRead MoreRenoirs Depection of Women in 19th Century Art2089 Words   |  9 PagesRenoir’s Depiction of Women in 19th Century Art The societal view of men verses women depicted in artwork during the nineteenth century differs from today’s view on the same subject. Pierre-Auguste Renoir (1841-1919), an impressionist painter in the European Arts, began his career in the early 1860’s. Many of the impressionistic painters of this time focused their paintings on landscapes, flower pieces and still-life works. Renoir’s work was mainly focused on portraits, largely female portraitsRead MoreANALYSIS OF ANGELICA KAUFFMAN ‘TELEMACHUS ON HIS RETURN TO HIS MOTHER’ 1770-1780 OIL ON CANVAS 1325 Words   |  6 PagesEngland’s John Locke who advanced the concept of â€Å"empiricism†. This denotes that accepting knowledge of matters of fact descends from experience and personal involvement1. Locke’s concept assisted the improvements of microscopes and telescopes allowing art students in the French academy to observe real life1. Science and experience influenced painti ng more so in Neo-Classicalism. Locke fought for people’s rights and the power or â€Å"contract† between the ruler and the ruled. Reasoning that â€Å"the Light inRead MoreThe Discovery Of America By Jan Van Der Straet1987 Words   |  8 Pagesrelationship between the Europeans of the Old World and the indigenous people of the New World. Exoticism, anxiety, and absurd speculation would fuel the European knowledge of the Americas during the late 15th and early 16th centuries. The drawing titled America by Jan Van der Straet, is a classic example of how Europeans used outlandish notions about indigenous Americans to distance themselves from these natives and thus, establish European superiority. Ultimately, Jan van der Straet’s image supports and justifiesRead MoreImpressionism And Its Impression On Modern Art1181 Words   |  5 PagesAnd its Impression on Modern Art One of the earliest forms of communication and documentation was art, from cave paintings, to Egyptian Hieroglyphs, to Renaissance portraits. Throughout the beginning of time, artistic style has evolved, and is still continuing to evolve. One early impact on Modern Art was Impressionism. Impressionism was defined as French nineteenth century art movement between 1867 and 1886 that marks the momentary break from tradition in European painting. In the middle of the